Standarization of protocol for in Vitro regeneration of mint (mintha sp.)

Abstract

Abstract : An attempt was made to standardize the protocol for in vitro regeneration of mint at the Tissue Culture Laboratory of the Bangabandhu Sheikh Mujibur Rahman Agricultural University during December 2012 to June 2013. Four levels of media (viz., M,= Full strength MS media without growth regulators, M3= Half strength MS media without growth regulators, M,= Full strength MS media supplemented with growth regulators, and M = Half strength MS media supplemented with growth regulators) along with three types of explant (viz., E,= Shoot tip, E = Node, and E = Petiole with a portion of leaf) were used in the experiment. Highest success (100%) in shoot regeneration was recorded in M,E„ M,E3, M,E„ M,E„ MJE„ and M4E3 while, the treatment combination M,E„ and M4E3 produced no shoot in vitro. On the other hand, highest success (100%) in root generation was recorded in M4E„ M,E„ and M,E3. Shoot tip and nodal segment along with full strength MS media supplemented with 1.0 mg/L of NAA and BAP were found to perform best in number of shoots, length of longest root (cm), number of visible intemodes, number of roots and length of longest shoot (cm) formation. On overall consideration, shoot tip and nodal segment along with full strength MS media supplemented with growth regulators (lmg/L NAA and lmg/L BAP) may be used in the case of in vitro regeneration of mint.