Pathological investigation of fowl typhoid in chickens in mymensingh

Abstract

Abstract : The present study was conducted to determine the prevalence of Salmonella Gallinarura in alive and dead chickens in Mymensingh district of Bangladesh. A total of 125 samples which included 65 swab samples (20 from liver, 15 from ovary and 30 from intestine) of dead layer chickens suspected to Fowl typhoid (FT) and 60 from cloaca! swabs of alive layer birds were attempted for isolation of bacteria. Routine methods of bacterial cultures in different media. Gram’s staining techniques and different types of biochemical tests were used for the isolation and identification of Salmonella Gallinarum. Routine histopathological method was used for the detection of tissue level alterations in fowl typhoid infected cases. Out of 125 samples, 8 isolates (6.4 %) which included 5 from dead birds (7.69%) and 3 from live birds (5%) were identified as Salmonella Gallinarum. Grossly, the liver was enlarged and congested and liver revealed bronze discoloration with focal necrosis. Old raised hemorrhages were found in the caecal tonsil. Pericarditis with turbid yellow fluid in the pericardial sac and fibrin attached to the surface of the heart. Congested, deformed, and pedunculated ova were other important findings. There was catarrhal inflammation in the intestine. Microscopically, the section of liver showed congestion and multifocal necrosis with infiltration of heterophils and RE cells. In ovary, different shaped ova with severe congestion and inflammation of egg yolk capsules were found. In heart, pericarditis and myocarditis were characterized by infiltration of RE cells, plasma cells and lymphocytes. Caecal tonsil showed severe hemorrhagic inflammation with infiltration of heterophils