Effect of different wheat bran supplements and mother cultures on growth and yield of oyster mushroom

Abstract

Abstract : Present study was conducted in the laboratory and culture-house of Mushroom Development Institute, Savar, Dhaka during September 2015 to August 2016 with a view to determining the effect of wheat bran as a supplement with rice straw and saw dust substrate and two types of mother cultures on the growth and yield of oyster mushroom {Pleurotus ostreatus). The substrates were prepared by mixing rice straw and saw dust at 1:1 ratio, which were then supplemented with wheat bran at a rate of 5,10,20, and 30%. The substrates were sterilized in a Sterilization Cum Inoculation Chamber. Sterilized and supplemented substrates were inoculated with jute stick or rice grain based mother culture and shifted to spawn running room. The highest mycelium run rate of 0.48 cm/ day was recorded in substrates supplemented with 30% wheat bran and inoculated with jute stick based mother culture, where a minimum of 20 days was required to complete mycelium running. On the other hand, the lowest mycelium run rate of 0.26 cm/day was observed in substrates supplemented with 5% wheat bran and inoculated with rice grain based mother culture, where a maximum of 34 days were required to complete the mycelium running. The highest biological yield (235.80 g/packet), economic yield (223.50 g/packet), biological efficiency (134.70 %) and benefit cost ratio (2.41) were recorded in substrate supplemented with 30% wheat bran and inoculated with jute stick based mother culture. Substrate supplanted with 30% wheat bran and inoculated with jute stick based mother culture is considered as the best strategy to achieve the highest biological yield, economic yield, biological efficiency and benefit cost-ratio.