In vitro shoot regeneration of mint {mentha sp.) using different explants and genotypes

Abstract

Abstract : An experiment was conducted in the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during March 2013 to January 2014 to observe the performance of nine mint genotypes (viz. MP-1, MP-2, MP-3, MP-4, MP-5, MP-6, MP-9, MP-19 and MP-22) with three explants (viz. leaf, shoot tip and node) for in-vitro shoot regeneration. In this experiment, two growth regulators BAP (1 mg/1) and NAA (1 mg/1) were used for shoot regeneration. Results revealed that the highest number of shoot was produced in shoot tip (1.94) at 55 days after inoculation (DAI), which initiated shoot within 20.09 days. In case of genotype, MP-1 showed the early response for shoot initiation with the highest shoot length but MP-4 (2.44) showed the better performance for number of shoot at 55 DAI. The interaction of MP-1 and node explant produced the highest number of shoots (4.66) at 55 DAI but initiated shoot within 9.67 days. Nodal explants showed the best result for the longest intemode (0.98 cm) at 55 DAI. Shoot tip and nodal segment was found the best explants in mint for zn vitro regeneration of shoot and the genotype MP-1, MP-4 and MP-6 performed better for shoot regeneration in vitro.