| dc.description.abstract | Abstract :
Chinese fever vine (Paederia foetida L.), a valuable medicinal plant has been greatly
utilized in therapeutic purposes throughout the world. Since conventional propagation
techniques of P. foetida are very slow, inefficient and cannot cope with the increasing
demand, in-vitro regeneration through tissue culture could be an alternative means
of rapid propagation. Therefore, the efforts were made to develop a suitable protocol
through direct organogenesis of P. foetida. After surface sterilization, the nodal explants
were cultured in Murashigue and Skoog (MS) medium and MS medium supplemented
with different concentrations and combinations of plant growth regulators. MS medium
supplemented with 6-benzylaminopurine; BAP (2.0 mg L'1) produced the maximum
number of shoots; 4.40 ± 0.98 and 5.40± 1.12 after 15 and 30 days of culture respectively.
The number of shoots gained by 15 days was found to be the highest; 1.20±0.80 at BAP
(4.0 mg L*1) followed by 1.00±0.55 at BAP (2.0 mg L*1). Although the combination of
BAP + Kinetin (2 mg L'1 +2 mg L1) showed the highest shoot growth (3.40 ± 1.08 cm)
by 15 days, sole application of BAP (2.0 mg L'1) or Kn (0.5, 1.0, 2.0 and 3.0 mg L1)
showed similar responses. BAP (2.0 mg L’1) showed the best responses for developing
the highest number of leaves; 18.60 ± 2.42 and 29.20 ± 2.73 respectively after 15
and 30 days of culture. Similarly, development of the maximum number of leaves
(10.60 ± 0.68) was reported by 15 days at BAP (2.0 mg L*1). Rooting was significantly
induced in indole-3-acetic acid (IAA) supplemented to 1/2 strength MS medium as
compared to control (only Vi strength MS medium). The best performance of rooting
was observed by 0.5 mg L"1 IAA which produced average 4.33 roots per shoot after
21 days of culture. The regenerated plants showed similar morphology to the mother
plants. Thus, a suitable protocol for successful multiplication of P. foetida in vitro was
established using nodal explants.
Keywords: Explant, regeneration, organogenesis.
Introduction
Chinese fever vine (Paederia foetida L.)
belonging to the family Rubiaceae is widely
distributed throughout the Asia. The plant
has been greatly used as the potential source
of traditional medicine specially in Asian
sub-continent. It has been very ubiquitous
that medicinal plants offer a great role across the varied cultures and civilizations throughout the world as a valuable and safe natural resource. Medicinal plants are being considered as the most important source of life saving drugs for the majority of world’s population (Kumar and Gupta, 2008). | en_US |
